Agarose
Agarose is a polysaccharide formed by β-D-galactopyranose units linked to 3,6-anhydro-α-L-galactopyranose units via 1-4 bonds. This basic repeating unit, called agarobiose, forms long chains. This structure contains charged groups, such as sulphate and pyruvate, and uncharged groups, all of which are responsible for many of the properties of agarose. These properties can be adjusted to meet specific needs through careful selection of the raw material.
Some important properties of agarose are:
Gel strength: the force required to break a gel, expressed in g/cm².
Gelling temperature: the temperature at which an agarose solution forms a rigid gel.
Melting temperature: the temperature at which the gel becomes a solution.
EEO (electroendosmosis): a measure of the number of charged groups present in agarose.
Standard agarose is ideal for routine rapid separation of DNA and RNA fragments, as well as for PCR products, plasmid preparation, and detection, cloning, and transfer techniques.
This is a highly purified agarose with low electroendosmosis values ranging from 0.05 to 0.13. Suitable for blotting assays. No DNase or RNase detected. Available with GQT (Genetic Quality Tested) quality control certificate. It is especially recommended for preparative DNA gels.
Biomax is an agarose ideal for rapid routine separation of DNA and RNA fragments, as well as for PCR products, plasmid preparation, and detection, cloning, and transfer techniques.
Agarose with a higher gelation temperature than D1 agaroses, providing greater thermal stability to gels. It has low electroendosmosis (≤ 0.14). Its main applications are nucleic acid electrophoresis, protein electrophoresis, and agarose bead preparation. There are low-viscosity variants for very high-concentration resins.
It is a linear polymer with a very high molecular weight, which means that, unlike traditional agaroses, it forms gel structures. This characteristic, combined with a very low sulphate content, produces a strong interchain interaction, resulting in a gel with very high gel strength and a high exclusion limit.
Low melting point (LM) agaroses are obtained by modifying their original natural chemical structure. The main properties of these agaroses are their low melting and gelling temperatures compared to standard agaroses.
LM
Low Melt agarose (with low melting point) recommended for the separation of fragments ≥ 1,000 bp in analytical electrophoresis. The low melting temperature (65 °C) allows the agarose to melt without damaging the DNA double helix. No DNases or RNases detected. SLM and ELM versions available with even lower melting points and GQT (Genetic Quality Tested) quality control certification.
LMSI
Very low melting temperature and high resolution capacity for fragments ≤ 1,000 bp, especially for PCR products between 200 and 800 bp with GQT (Genetic Quality Tested) quality control certification. No DNase or RNase detected.
NUGQ
Low melting and gelling temperatures, also GQT (Genetic Quality Tested) certified. This agarose, with high resolution capacity, separates fragments between 50 and 1,000 bp, especially in PCR products. No DNase or RNase is detected.
These agaroses have been chemically modified to obtain a structure that improves the separation of small DNA fragments and the resolution between fragments of very similar sizes.
MS04
The agarose with the highest molecular sieving capacity; capable of separating very small fragments with differences of only a few base pairs. Its gelation/melting temperatures are in the range of low-melt agaroses. Its low viscosity allows high-concentration gels to be prepared, thereby increasing its sieving capacity.
MS06
It offers improved resolution efficiency for small DNA fragments (10-1,200 bp) and PCR products. No DNases or RNases are detected, and it has very low DNA binding.
MS08
Recommended product for analytical DNA gels ≤ 1,200 bp and especially PCR products. No DNase or RNase is detected and it does not bind DNA.
MS12
Recommended for analytical DNA gels. At a concentration of 2%, it is capable of separating fragments of 30-1,500 bp. No DNases or RNases are detected, and it does not bind DNA.