Protein Hydrolysates
Peptones are hydrolysed proteins of animal or vegetable origin. The main application of peptones is the production of culture media, whether dehydrated or prepared. They are also used in the production of media for the fermentation of plant tissues, as stabilisers in vaccines, cosmetics, electroplating and dietary products. These products provide an effective source of nitrogen and carbon, as well as other essential nutrients. When used in appropriate formulations, they guarantee and intensify the growth of microorganisms. Numerous peptones, extracts and infusions from protein sources are used, among other applications, as nutrients in microbiological and tissue culture media and in vaccine development.
Plant-based peptones with low endotoxin levels that improve cell density and protein production in eukaryotic and prokaryotic cell cultures in BioPharma processes. Endolow® Soy Peptone is a product free of genetic modifications and animal derivatives obtained through the controlled enzymatic digestion of soy protein, ensuring low endotoxin levels. EndoLow Wheat Peptone is an animal derivative-free protein hydrolysate based on wheat protein with low endotoxin content.
Low-endotoxin peptones that improve cell density and protein production in eukaryotic and prokaryotic cell cultures in BioPharma processes. EndoLow® Meat Peptone is produced by controlling the enzymatic digestion of animal tissue to ensure low endotoxin levels.
Low-endotoxin peptones that improve cell density and protein production in eukaryotic and prokaryotic cell cultures in BioPharma processes. EndoLow® Casein Peptone is a pancreatic digest of casein. The controlled manufacturing process ensures low endotoxin content. CH Endolow® Casein Peptones are also available.
Casein peptones are casein hydrolysates that provide soluble peptides and amino acids used as a source of carbon and nitrogen in culture media and biotechnological processes. depending on the method of production, there are variants with specific properties and uses: Acid Casein Peptone, obtained by acid hydrolysis, destroys amino acids such as glutamine, asparagine, tryptophan, cysteine, serine, threonine, lysine, aspartic acid and proline, and completely eliminates vitamins. As it is a vitamin-free peptone, it is used in the microbiological determination of vitamin content and as a raw material for preparing agar and Müller–Hinton broth thanks to its good solubility and clarity. casein peptones obtained by pancreatic digestion include Type I, which contains all the amino acids present in casein together with larger peptide fractions and is an excellent nutrient for media intended for the production of antibiotics, toxins, enzymes and other biological products, widely used in the pharmaceutical, veterinary and diagnostic media industries; Type II, suitable for the manufacture of toxins, vaccines and enzymes and especially suitable for media containing blood; Type III, which in addition to providing all amino acids and large peptide fractions has particular digestion properties and a high phosphate content that makes it the choice of many organisms; Type III, which in addition to providing all amino acids and large peptide fractions, has particular digestive properties and a high phosphate content that makes it the choice of many organisms; Type V, a white powdered pancreatic digest widely used as a bacteriological nutrient due to its excellent solubility and clarity in solution; and Type VI, also a pancreatic digest that is a highly soluble source of peptides and amino acids, recommended for fermentations and the manufacture of toxins, vaccines and enzymes, as well as for use as a nutrient in laboratory media, whose 2% solution is clear and free of precipitation. In addition, there are bacteriological casein peptones noted for their high quality, produced by enzymatic digestion of animal tissues and widely used in culture media and in the production of toxins, vaccines and other biological products; among these, type II bacteriological casein peptone is used in fermentation processes where clarity and transmittance are not essential and is used in culture media for diagnostic testing and bacteriological research, as well as in industry for the production of antibiotics, vaccines and other biological products.
Animal-derived peptones are protein hydrolysates obtained by enzymatic or proteolytic digestion of animal tissues that provide soluble peptides and amino acids used as sources of carbon and nitrogen in culture media, fermentations, and biotechnological production. Among them, gelatin peptone is a pancreatic digest of pig skin with low fermentable carbohydrate content, suitable for non-demanding microorganisms and fermentation studies, while lactalbumin hydrolysate, obtained by pancreatic digestion of lactalbumin and whey protein, is notable for its high content of essential amino acids and is used in microbiological media formulations and tissue culture. Meat peptones, whether bovine or porcine, are enzymatic digests of animal tissues that can be incorporated into a wide variety of liquid and solid media formulations for the culture of demanding and non-demanding microorganisms, with meat peptone obtained by proteolytic digestion with pepsin being a common choice for many microorganisms and pork peptone being interchangeable with beef peptone in many formulations; Proteose peptones are high-quality hydrolysates produced by enzymatic digestion of animal tissues and are used in culture media and in the production of toxins, vaccines and other biological products. General proteose peptone is used to manufacture media for the production of bacterial toxins and for initial fermentation cultures adapted to bacteria with different nutritional requirements. Proteose Peptone No. 3 is noted for its quality for bioproductive uses, and Proteose Peptone No. 5, a pancreatic digest of animal tissue and yeast, is recommended as an ingredient in media for non-fastidious microorganisms and in fermentation studies. Finally, porcine heart digest is an enzymatic hydrolysate of heart tissue used in the production of commercial media such as Columbia Agar or nutrient broths.
The other peptones comprise a variety of hydrolysates and mixtures designed for specific applications in microbiology and fermentation. For example, biotriptase, produced from animal and plant proteins, is ideal for general cultivation and especially recommended for species of the genus Brucella. Type C fermentation peptone is a formulation that combines peptones, salts and dextrose and is used in fermentation processes where clarity and transmittance are not essential; mycological peptone, the result of mixing plant and animal peptones, is designed for the isolation and diagnosis of pathogenic and non-pathogenic fungi, promoting rapid and exuberant growth; Peptonised milk is a pancreatic digest of skimmed milk that is mainly used in media for the isolation and growth of lactobacilli and streptococci from dairy products. and there are specific mixtures of casein peptone and meat peptone that act as a superior nutrient source in culture media, in some cases promoting better growth than peptones used separately and being especially useful for the development of enterobacteria; together, these peptones complement the supply of nutrients available to adapt media to growth, selectivity and performance requirements in research, diagnosis and industry.
Peptones of plant origin are hydrolysates of plant proteins that provide soluble peptides and amino acids used as sources of carbon and nitrogen in microbiological and industrial culture media and in fermentation processes. Among them, wheat peptone is a hydrolysate derived from wheat gluten protein, which is digested using non-animal enzymes. It is suitable for both microbial broths and solid media and is useful in applications where high clarity is not required. Soy peptone, produced by digestion with papain from defatted soy flour, is a product rich in essential amino acids and vitamins that is used in culture media and process formulations; Type III Soy Peptone, also obtained by enzymatic digestion with papain from defatted soy flour, is used in fermentation processes where clarity and transmittance are not essential; GMO-free & animal-free soy peptone, also produced by digestion with papain from defatted soy flour, guarantees the absence of genetic modification and animal derivatives and is recommended for dehydrated culture media, tissue culture and fermentation processes; and marine peptone is a purified extract obtained by autolysis of fresh marine raw materials and supplemented with yeast extract to offer a substitute with high nutritional value compared to animal tissue digests.
Extracts are products obtained from animal and plant protein sources through separation processes. They are used in the formulation of a wide variety of bacteriological culture media due to their high nutritional value.
Beef extract powder is prepared from bovine skeletal muscle with very low fat content and tendon-free marrow. The result is a highly nutritious extract used in the preparation of microbiological culture media. It is recommended for use in the bacteriological examination of water, milk and other materials.
This is a water-soluble extract from selected autolysed yeast cells, a rich source of vitamins and growth factors. It is used in veterinary products and in various fermentation processes. These products are obtained by cooking animal or plant nutrients in hot water to extract their nutrients. They are used in a wide variety of bacteriological culture media.
These products are obtained by cooking animal or plant nutrients in hot water to extract their nutrients. They are used in a wide variety of bacteriological culture media.
Preparation supplied as a light fine powder, specially formulated with dry extract of bovine hearts and brains. Used in microbiological culture media.
Specially formulated with dry extract of pig hearts and brains. It is a suitable substitute for Heart-Brain Infusion (bovine) in selected culture media.
Specially formulated with dried bovine heart infusion, supplied in fine powder form for use in microbiological culture media.
Formulated with dry infusion of pig hearts, supplied in the form of a fine powder. It is a suitable substitute for Heart Infusion (bovine) in microbiological culture media.
Beef Infusion is a product specially derived from lean beef, supplied as a light beige powder that is used for the cultivation of demanding microorganisms.
This product is prepared using a low-temperature dehydration process. It is used as a selective inhibitor agent in culture media such as Brilliant Green Bile 2% broth.
Partially hydrolysed protein obtained from the alkaline hydrolysis of animal skin and connective tissue.
This group includes products used in the preparation of a wide variety of bacteriological culture media that may be specific or selective for the identification of microorganisms.