{"id":1938,"date":"2021-02-10T11:22:00","date_gmt":"2021-02-10T10:22:00","guid":{"rendered":"https:\/\/www.hispanagar.com\/blog\/consejos-o-pasos-para-correr-una-electroforesis\/"},"modified":"2026-04-23T07:19:33","modified_gmt":"2026-04-23T05:19:33","slug":"tips-or-steps-to-run-an-electrophoresis","status":"publish","type":"post","link":"https:\/\/www.hispanagar.com\/en\/life-sciences\/tips-or-steps-to-run-an-electrophoresis\/","title":{"rendered":"Tips or steps to run an electrophoresis"},"content":{"rendered":"<p><span><span><span><span><span><span><span><span>Before we start, we need to ask ourselves a series of questions: <\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<p><span><span><span><span><span><span><span><strong><span>What do I want to separate with the&nbsp;<\/span><\/strong><strong><span><span>electrophoresis<\/span><\/span><\/strong><strong><span>?&nbsp;<\/span><\/strong><span>As we know, the&nbsp;<\/span><span><span>electrophoresis<\/span><\/span><span>&nbsp;is a separation technique, so knowing what I want to separate is essential.&nbsp;<\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<p><span><span><span><span><span><span><span><strong><span>What size are the molecules\/fragments&nbsp;I want to separate?&nbsp;<\/span><\/strong><span>The size of the molecules\/particles to be separated&nbsp;is crucial when choosing the appropriate agarose.&nbsp;<\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<p><span><span><span><span><span><span><span><strong><span>What do we want to do afterwards with the molecules separated in the electrophoresis?<\/span><\/strong><span>&nbsp;The goal of the&nbsp;<\/span><span><span>electrophoresis<\/span><\/span><span>&nbsp;can be just to visualize the separation of the molecules\/fragments or to recover them for further manipulations&nbsp;&nbsp;<\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<p><span><span><span><span><span><span><span><strong><span>What <a href=\"https:\/\/www.hispanagar.com\/es\/productos\">type of agarose<\/a> should I use?<\/span><\/strong><span>&nbsp; The type of agarose to be used will be determined by several factors, including the nature and size of the molecule we want to separate.<\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<p><span><span><span><span><span><span><span><span><span>Once we have determined what we want to separate, its size, and which agarose we should use, we need to set the parameters of the&nbsp;<\/span><span><span>electrophoresis<\/span><\/span><span>:<\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<ul>\n<li><span><span><span><span><span><strong><em>Concentration<\/em><\/strong><em>&nbsp;<\/em>of the gel&nbsp;<\/span><\/span><\/span><\/span><\/span><\/li>\n<li><span><span><span><span><span><strong><em>Buffer<\/em><\/strong><strong>r<\/strong>&nbsp;that we must use&nbsp;<\/span><\/span><\/span><\/span><\/span><\/li>\n<li><span><span><span><span><span>Specific conditions of the&nbsp;<span>electrophoresis<\/span>&nbsp;such as&nbsp;<strong><em>time and voltage<\/em><\/strong><\/span><\/span><\/span><\/span><\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Before we start, we need to ask ourselves a series of questions: What do I want to separate with the&nbsp;electrophoresis?&nbsp;As we know, the&nbsp;electrophoresis&nbsp;is a separation technique, so knowing what I want to separate is essential.&nbsp; What size are the molecules\/fragments&nbsp;I want to separate?&nbsp;The size of the molecules\/particles to be separated&nbsp;is crucial when choosing the appropriate [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":1356,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[29],"tags":[],"class_list":["post-1938","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-life-sciences"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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